Background/Aims: Many stroke survivors live with permanent upper limb disability. While motor training improves strength, coordination, and limb use, recovery may be further enhanced by adjuvant therapies that promote a neuroplastic environment. This systematic review aimed to determine if task-specific forelimb motor training either alone or in combination with other interventions, improves performance in forelimb motor tasks or enhances generalised motor recovery after ischaemic stroke in animal models.
Method: Searches were conducted in PubMed, MEDLINE, Embase and Web of Science. Two authors independently screened titles and abstracts, reviewed full-texts and extracted data.
Results: Fifty-nine studies were included, most were conducted in rats (n=49, 83%) using predominantly male animals (n=46, 78%) with endothelin-1 (n=31, 53%) and photothrombosis (n=18, 31%) models most common. Adjuvants were evaluated in 68% of studies and included cortical stimulation, vagus nerve stimulation, and pharmacological, behavioural, and molecular therapies. Motor training improved performance in the single pellet reaching task (SMD 1.53, p<0.00001) and pasta matrix reaching task (SMD 0.79, p=0.0001), but not performance on the staircase (reaching), cylinder (spontaneous forelimb use), or grid/ladder walking tests (locomotion and limb placement). Cortical stimulation, enriched environment, and dietary interventions significantly improved reaching performance compared to motor training alone.
Conclusion: Emerging adjuvants (e.g. cortical stimulation, enriched environment) show promise as translational candidates to enhance post-stroke recovery. Clinically, there is growing interest in combining adjuvants with motor training. These findings identify promising candidates to inform future clinical trial design.